#Bioedit reverse complement download
You can use, search and download the sequence.īy default the FASTA search page is set up for protein similarity search. Since we have a universal accession number we can use all major portals. Try yourself first, click 'Show' for tips. Get the sequence with accession number M25165 in fasta, download it and save as M25165.fasta. (Note:running against the complete EMBL databank as compared to the bacteriophage section would take to much time for this exercise session - remember that fasta is rather slow). We will search with a fragment of the bacteriophage lambda genome containing the third right operator (OR3) and the right promoter (PR) to find similar sequences in the bacteriophage section of EMBL databank. Go to, then select the menu item "Tools"/"Similarity & Homology"/"FASTA". We will use the FastA server of the EMBL-EBI, which has a richer collection of databanks. FastA is older than the popular BLAST, it is slower, but sometimes finds things that BLAST does not find, especially for non-coding DNA (e.g. Pearson's FastA finds similar sequences in a database given a query sequence.
Then you can make the exercises below in any order you want.įastA at the EMBL-EBI Introduction of FastA These exercises are additional to the basic exercises: first make sure you have made all basic exercises. 1.11 Advanced multiple sequence alignmentĪdditional exercises on sequence similarity search.1.8 Nice graphical comparison of two sequences, using SIM6 and LALNVIEW.
1.7 The sequences that will save your scientific life.1.4 Dotter, a small useful tool to quickly visually compare two sequences.1 Additional exercises on sequence similarity search.